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There isn't a proof to recommend that Glu-131 plays any function in catalysis besides to assist coordinate the single divalent cation. The enzyme is fee confined from the cleavage from the amide bond.
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At the moment 1.five mL of acetic anhydride was added and allowed to respond at space temperature overnight. Right after removal with the solvent, the residue was dissolved in a hundred mL of chloroform and washed with dilute HCl, saturated NaHCO3 and after that dried around Na2SO4. The product, diphenyl N
Mutation of this residue to either glutamine or alanine leads to mutant enzymes which have missing an important quantity of catalytic activity. The value of k
there are actually tyrosine (Y223) and lysine (K139) residues that may also be contributing into the interaction Together with the phosphate moiety from the substrate. Deprotonation of possibly of these residues at large pH could lead to a drop in the worth of k
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